CRISPR/Cas9-mediated homology-directed repair by ssODNs in zebrafish induces complex mutational patterns resulting from genomic integration of repair-template fragments
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biologists.com
Mechanisms of DNA Repair
Mechanisms of DNA Repair
Mar 6, 2018
news-medical.net
CRISPR-Cas mediated homology-directed repair (HDR) allows for precise genome modifications, but achieving high HDR efficiency can be challenging in some cells. By conducting a genome-wide screening in Fanconi anemia patient cell lines, researchers identified TREX1, an exonuclease, as a key factor that reduces HDR efficiency when linear DNA is used as a repair template. High TREX1 levels are linked to poor HDR, making it a biomarker for HDR efficiency. Strategies such as TREX1 removal and chemica
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CRISPR-Cas mediated homology-directed repair (HDR) allows for pr…
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