Definition: A restriction enzyme is a DNA-cutting protein, found mainly in bacteria and archaea, that recognizes a specific short DNA sequence and cleaves the double helix at or near that site. By ...
In the early 1970s, researchers reported that restriction enzymes cleave DNA at specific sites, producing individual fragments that are distinguishable using gel electrophoresis. 1,2 This timely ...
Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...
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